Production of CGTase from Bacillus subtilis - Bhargavi
Kina Alpha Cyclodextrin CAS-nr 10016-20-3
Bars represent extracellular γ-CGTase activity (black), soluble periplasmic γ-CGTase activity (light gray), and DCW (gray). Efficient synthesis of a long carbohydrate chain alkyl glycoside catalysed by cyclodextrin glycosyltransferase (CGTase). Svensson, David LU; Ulvenlund, Stefan and Adlercreutz, Patrick LU () In Biotechnology and Bioengineering 104 (5). p.854-861 A novel CGTase (Cyclomaltodextrin glucanotransferase) has been isolated from a strain of Thermoanaerobacter, a thermophilic anaerobe. The enzyme is extremely heat stable and has a temperature optimum of 90-95°C at pH 6.0. It is active over a broad pH range, and exhibits more than 80% activity from pH 5.0-6.7.
Further experiments demonstrated that CGTase is a potential func - Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is a carbohydrate‐active enzyme that belongs to glycosyl hydrolase family 13. CGTase can catalyse hydrolysis reactions, cleaving the linkages in the starch molecule (van der Veen et al., 2000). Publisher Summary This chapter discusses the purification and action of cyclodextrin-producing enzyme (CGTase). Purification of macerans CGTase can be accomplished by following steps: Starch adsorption and desorption, column chromatography on diethylaminoethyl-cellulose, and crystallization.
Efficient synthesis of a long carbohydrate chain alkyl glycoside catalysed by cyclodextrin glycosyltransferase (CGTase).
Cloning and expression of a putative cyclodextrine
2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely cyclization, disproportionation and coupling.
Göra Cyclodextrin - Flashback Forum
1996-01-01 2009-07-08 Cyclodextrin glycosyl transferase (CGTase, EC 2.4.1.19) is an important industrial enzyme, unique in its ability to convert starch and related glycans into non-reducing, cyclic malto-oligosacchrarides called cyclodextrins (CDs) via a cyclization reaction, an intramolecular transglycosylation reaction [1].
CGTase, also known as cyclodextrin glycosyltransferase and cyclodextrin glucanotransferase, catalyzes the formation of various cyclodextrins (alpha-1,4-glucans) from starch. CGTase has, in addition to its C-terminal CBM20 domain, an N-terminal catalytic domain belonging to
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond. reaction in four steps via cyclization, disproportionation, hydrolysis, and coupling. The second step of the reaction involves the transfer of the covalently bound saccharide to an acceptor molecule.
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CGTases produced by Bacillus stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. CGTase from different microorganisms has different numbers of residues in 5 domains (A-E). CGTase from Bacillus circulans strains 8 has 80–108 residues in domain A, 185–192 residues in domain B, 407–494 residues in domain C, 495–580 residues in domain D and 581–684 residues in domain E . Cyclodextrin glucanotransferase [CGTase, E.C.2.4.1.19] is an extracellular enzyme, which catalyzes the formation of α−, β−, γ− CDs from starch.
and B. circulance during growth phase
2014-03-13
cgtase.
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rekombinant - English translation – Linguee
Unreviewed-Annotation score: -Protein inferred from homology i. Function i Cofactor i. Ca 2+ ARBA annotation. GO - Molecular function i.
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rekombinant - English translation – Linguee
Organism. Anaerobranca gottschalkii.